USP 621 Compliance with the system suitability criteria is required throughout the chromatographic procedure. No sample analysis is acceptable unless the suitability of the system has been demonstrated.

原则上SST满足要求后的测试才有效果，需要对于方法验证的数据进行评估，评估方法验证数据的有效性，根据评估的结果确定是否需要进行重新验证。